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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 2: Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Topic 3: Blood Banking | 15–20% | - Blood products and components - Blood group systems and immunology - Transfusion practice and testing |
| Topic 4: Immunology | 5–10% | - Principles of immunology - Serologic procedures and immune diseases |
| Topic 5: Microbiology | 15–20% | - Virology and parasitology testing - Bacteriology and mycology procedures |
| Topic 6: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 7: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Which of the following CD markers is NOT present on a normal mature T cell?
- A. CD7
- B. CD20
- C. CD3
- D. CD5
Correct Answer: B 🗳️
The DAT, or direct antiglobulin test, is used to detect antibodies coating RBC's in vivo.
The Direct Antiglobulin Test (DAT) is usually used to detect:
- A. Antibody-hapten reactions
- B. Antibodies in the plasma
- C. Antibodies coating red cells
- D. Antigens in the plasma
- E. Antigens coating red cells
Correct Answer: C 🗳️
Which area of the laboratory is responsible for blood coagulation studies that test for the patient's ability to clot their blood?
- A. Immunology
- B. Hematology
- C. Blood banking/transfusion medicine
- D. Clinical chemistry
Correct Answer: B 🗳️
The negative antigen frequencies are used in the formula below to determine the percent of type-specific units that would be compatible for the patient. Negative antigen frequencies are determined by subtracting the percent antigen frequency from 100%. The negative antigen frequency for K in this case is .91 (1.00 - .09) and the negative antigen frequency for Fya is .34 (1.00 - .66).
% compatible units available = 100 x (Neg frequency #1 x Neg frequency #2...) For this case, the calculation is:
% compatible units available = 100 x (0.91 x 0.34) = 30.94 or 31%
If the antigen frequencies for K = 0.09 and Fya = 0.66, what percent of type-specific units would be compatible for a patient with anti-K and anti-Fya?
- A. 100
- B. 10
- C. 31
- D. 92
- E. 43
Correct Answer: C 🗳️
HLA-DR is a MHC class II(major histocompatibility complex) surface receptor on the cellular surface which is encoded by the human leukocyte antigen complex, or HLA.
DR antigens are found in which of the following systems:
- A. HLA system
- B. Kell system
- C. Duffy system
- D. ABO system
Correct Answer: A 🗳️
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